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DNA

Part:BBa_K798001

Designed by: Taijie Jin   Group: iGEM12_Fudan_D   (2012-09-24)


600bp overlong telomere repeats of Saccharomyces cerevisiae

This part is a 600bp overlong telomere repeats of Saccharomyces cerevisiae(its general length is about 300bp). According to some researches before, the overlong telomere in the yeast will decrease by 2-3bp per generation. Thus, we can insert some key regulation sequence (but the sequence has to be very small, <100bp) in the overlong part of the telomere sequence. After recombination into the yeast chromosome, the telomere begins to decrease and about 70 generations later the key sequence will be cut off which can have an effect on the regulation. Obviously, the main advantage of the design is that we can change the regulation procedurally and predictably.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 601
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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